DDK Has a Primary Role in Processing Stalled Replication Forks to Initiate Downstream Checkpoint Signaling
نویسندگان
چکیده
منابع مشابه
Assembly of Slx4 signaling complexes behind stalled replication forks
Thank you for your patience with our evaluation of your manuscript on Slx4 signaling complexes on replicating DNA. We have now received the comments of all three referees, copied below for your information. As you will see, all referees generally acknowledge the interest and potential importance of this work, yet they also raise a number of critical issues regarding the experimental analyses an...
متن کاملContinued primer synthesis at stalled replication forks contributes to checkpoint activation
Stalled replication forks activate and are stabilized by the ATR (ataxia-telangiectasia mutated and Rad3 related)-mediated checkpoint, but ultimately, they must also recover from the arrest. Although primed single-stranded DNA (ssDNA) is sufficient for checkpoint activation, it is still unknown how this signal is generated at a stalled replication fork. Furthermore, it is not clear how recovery...
متن کاملThe Intra-S Phase Checkpoint Targets Dna2 to Prevent Stalled Replication Forks from Reversing
When replication forks stall at damaged bases or upon nucleotide depletion, the intra-S phase checkpoint ensures they are stabilized and can restart. In intra-S checkpoint-deficient budding yeast, stalling forks collapse, and ∼10% form pathogenic chicken foot structures, contributing to incomplete replication and cell death (Lopes et al., 2001; Sogo et al., 2002; Tercero and Diffley, 2001). Usi...
متن کاملRecG regresses stalled DNA replication forks
RecG and RuvAB are proposed to act at stalled DNA replication forks to facilitate replication restart. To clarify the roles of these proteins in fork regression, we used a coupled spectrophotometric ATPase assay to determine how these helicases act on two groups of model fork substrates: the first group mimics nascent stalled forks while the second mimics regressed fork structures. The results ...
متن کاملMultiple pathways process stalled replication forks.
Impairment of replication fork progression is a serious threat to living organisms and a potential source of genome instability. Studies in prokaryotes have provided evidence that inactivated replication forks can restart by the reassembly of the replication machinery. Several strategies for the processing of inactivated replication forks before replisome reassembly have been described. Most of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Neoplasia
سال: 2018
ISSN: 1476-5586
DOI: 10.1016/j.neo.2018.08.001